Anti-Ectonucleotide Pyrophosphatase, Phosphodiesterase 5 (E-NPP5, NPP-5, UNQ550/PRO1107)

Ectonucleotide pyrophosphatase/phosphodiesterase 5 (ENPP-5) belongs to a group of nucleotide-metabolizing ecto-enzymes, which regulate the availability of extracellular nucleotides. It may also be involved in neuronal cell communication. Applications: Suitable for use in Direct ELISA, Western Blot, and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Direct ELISA: 0.5-1ug/ml. The detection limit for recombinant human ENPP-5 is ~1ng/well, Immunoprecipitation: 25ug/ml will immunprecipitate recombinant human ENPP-5 from conditioned cell culture media, Western Blot: 1-2ug/ml. The detection limit for recombinant human ENPP-5 is ~5ng/lane under non-reducing and reducing conditions. Chemiluminescent detection will increase by 5 to 50 fold., Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.