Anti-E2F1 Transcription Factor, clone 3G138

E2F-1 is a transcription factor (437 amino acids, 46kD) that contains a cyclin A binding domain, a DNA binding domain, a dimerization domain, and a transactivation domain. E2F-1 is expressed in a wide variety of cell lines and tissues and is expressed broadly, but not uniformly, during mouse embryogenesis. E2F-1 binds almost exclusively to pRB and phosphorylation of E2F-1 may disrupt pRB/E2F complexes. Overexpression of E2F-1 or dE2F in normal cells causes high levels of apoptosis while p19 arf -de?cient cells are partially resistant to E2F-1-induced apoptosis. p53-independent apoptosis requires the E2F-1 DNA-binding domain but not an intact transactivation domain. Applications: Suitable for use in Western Blotting, Immunoprecipitation, Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry: 0.1-1.0ug/ml, Immunoprecipitation: 2-5ug/IP , Western Blotting: 1-2ug/ml , , Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.