Anti-Dok3 (Docking Protein 3, DOK Like Protein, DOKL, FLJ22570)

Downstream of kinase 3 (DOK3) is a 53kD (predicted) member of the DOK family of cytoplasmic adaptor proteins and is expressed in B cells and macrophages. It contains one pleckstrin homology region (aa63-179) and an IRS-type phosphotyrosine binding domain (PTB) (aa213-317). Alternate splicing generates additional isoforms with extensive N-terminal and C-terminal truncations and substitutions. Phosphorylation of tyrosine residues in DOK3 regulates its interaction with SH2 and SH3 containing proteins, leading to inhibition of signaling from immunoreceptors. Over (aa181-271), human DOK3 shares approximately 80% aa sequence identity with mouse and rat DOK3. Applications: Suitable for use Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 0.25ug/ml, Optimal dilutions to be determined by the researcher. Hybridoma: Myeloma cells with spleen cells from Balb/c mice. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.