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Item number | Size | Datasheet | Manual | SDS | Delivery time | Quantity | Price |
---|---|---|---|---|---|---|---|
137430.100 | 100 µl | - | - |
3 - 19 business days* |
757.00€
|
If you have any questions, please use our Contact Form.
You can also order by e-mail: info@biomol.com
Larger quantity required? Request bulk
You can also order by e-mail: info@biomol.com
Larger quantity required? Request bulk
DNA topoisomerase I and II are nuclear enzymes, and type II consists of two highly homologous... more
Product information "Anti-DNA Topoisomerase II (Topoisomerase II-alpha )"
DNA topoisomerase I and II are nuclear enzymes, and type II consists of two highly homologous isoforms: topoisomerase IIa and IIb. These enzymes regulate the topology of DNA, maintain genomic integrity, and are essential for processes such as DNA replication, recombination, transcription, and chromosome segregation by allowing DNA strands to pass through each other. Topoisomerase I nicks and rejoins one strand of the duplex DNA, while topoisomerase II transiently breaks and closes double-stranded DNA. Topoisomerases are very susceptible to various stresses. Acidic pH or oxidative stress can convert topoisomerases to DNA-breaking nucleases, causing genomic instability and cell death. DNA-damaging topoisomerase targeting drugs (e.g., etoposide) also convert topoisomerases to nucleases, and the enzyme is usually trapped as an intermediate, covalently bound to the 5+ end of the cleaved DNA strand(s). Research studies have shown that this intermediate leads to genomic instability and cell death, and thus, agents that target topoisomerases are highly sought after cancer chemotherapeutic drugs. Ca2+-regulated phosphorylation of topoisomerase IIa at Ser1106 modulates the activity of this enzyme and its sensitivity to targeting drugs. Applications: Suitable for use in Immunofluorescence/Immunocytochemistry, Flow Cytometry, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Immunofluorescence (IC): 1:1600, Flow Cytometry: 1:100, Western Blot: 1:1000. Incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight., Immunohistochemistry (Paraffin): 1:400. Use 10mM sodium citrate buffer, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Supplier: | United States Biological |
Supplier-Nr: | 137430 |
Properties
Application: | FC, IF, IHC, WB |
Antibody Type: | Monoclonal |
Clone: | 13G28(D10G9) |
Conjugate: | No |
Host: | Rabbit |
Species reactivity: | human, monkey |
Immunogen: | Synthetic peptide corresponding to residues near the C-terminal of human topoisomerase IIa. |
Format: | Purified |
Database Information
KEGG ID : | K03164 | Matching products |
UniProt ID : | P11388 | Matching products |
Gene ID | GeneID 7153 | Matching products |
Handling & Safety
Storage: | -20°C |
Shipping: | +4°C (International: -20°C) |
Caution
Our products are for laboratory research use only: Not for administration to humans!
Our products are for laboratory research use only: Not for administration to humans!
Information about the product reference will follow.
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