Anti-Dipeptidyl Peptidase 10 (DPP10)

DPP10 (dipeptidyl peptidase 10, also DPPY, DPRP3 and DPL2) is a 91-97kD member of the peptidase S9B family. It is a type II transmembrane glycoprotein principally expressed in neurons and T cells. Although it is a peptidase member, it has no catalytic activity. Instead, it assists in the membrane trafficking and functioning of Kv4 K+ channels. Human DPP10 is 796 amino acids (aa) in length. It contains a 34 aa cytoplasmic N-terminus and a 741 aa extracellular domain (ECD) (aa 56-796). Three potential isoforms exist, all involving aa substitutions limited to the first 20 amino acids. The ECD of human DPP10 shares 89% aa sequence identity with mouse DPP10 ECD. Applications: Western Blot: This antibody can be used at 0.1-0.2ug/ml with the appropriate secondary reagents to detect human DPP10. The detection limit for rhDPP10 is approximately 2 ng/lane under non-reducing and reducing conditions. , Direct ELISA: This antibody can be used at 0.5-1.0ug/ml with the appropriate secondary reagents to detect human DPP10. The detection limit for rhDPP10 is approximately 0.3 ng/well., Optimal dilutions should be determined by each laboratory for each application. Storage and Stability: Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile PBS with 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.