Anti-DC-SIGN (Dendritic Cells, CD209, Dendritic Cell-Specific ICAM-3, SIGNR1, SIGN-R1)

Dendritic cells (DC) are specialized antigen presenting cells and bridge the innate and the adaptive immune system. They provide high levels of costimulation necessary for activation of both naïve and antigenexperienced T-cells. Immature DC are capable to migrate to inflammatory sites, capture antigen and process it internally to form MHC-peptide complexes. Following antigen uptake, DC undergo maturation and migrate to lymphoid organs where they can present, MHC-peptide complexes to resting T-cells and drive T-cell proliferation. During differentiation and maturation of DC, several phenotypic surface markers are expressed: CD1a, CD4, CD11, CD40, CD86, and HLA-DR. Immature DC predominantly express CCR5 which enables DC to migrate to inflammatory sites, whereas mature DC express high levels of CXCR4, a receptor that facilitates migration to lymphoid organs. DC also express DC-specific, ICAM-3 grabbing, nonintegrin (DC-SIGN), a 44kD C-type lectin that binds to the HIV-1 envelope glycoprotein gp120, ICAM-3 on T-cells and ICAM-2 on endothelial cells. HIV virions are able to infect cells expressing CD4 and the chemokine co-receptors CCR5 or CXCR4 and can attach to DC-SIGN to extend virion lifespan. Therefore, DC are candidates for HIV-1 infection. DC-SIGN-ICAM-3 binding is integrin-independent but calcium-dependent and antibodies reactive against DC-SIGN can be used to study DC-SIGN-ICAM3 binding. The monoclonal antibody DCN47.5 specifically reacts with the C-type lectin DC-SIGN (CD209) expressed on human dendritic cells and inhibits binding of DC-SIGN to ICAM-2 on endothelial cells. , Applications:, Suitable for use in Flow Cytometry, and for functional studies on the binding of DC-SIGN to its, natural ligands. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:50, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.