Anti-Cyclooxygenase-2, CT (PTGS2, Prostaglandin G/H Synthase 2, COX-2, PHS II, Prostaglandin H2 Synt

Prostaglandin-endoperoxide synthase (fatty acid cyclooxygenase, PGH synthase, Cox-2) is a key enzyme in prostaglandin biosynthesis. The first rate-limiting step in the conversion of arachidonic acid to prostaglandins is catalyzed by Cox-1 and Cox-2. The cyclooxygenase activity of Cox-2 is inhibited by nonsteroidal antiinflammatory drugs such as aspirin and endomethacin. It is a mitogen-inducible gene (unlike Cox-1, which is a house-keeping gene), and this means it probably is a primary mediator of the inflammatory response. Applications: Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry (Formalin fixed paraffin embedded): 1:50, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.