Anti-Cyclin D3, clone 0.N.242

Cyclin D3 is a G1 cyclin closely related to cyclin D1 and D2. Cyclins D1, D2, and D3 form active kinase complexes in vivo with a distinct subset of cdks, particularly with cdk4 and cdk6. Applications: Suitable for use in Flow Cytometry, Immunofluorescence, Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications have not been tested. Recommended Dilutions: Immunoprecipitation (Native): 2ug/mg protein lysate. Use Protein G, Western Blot: 1-2ug/ml for 2 hrs at RT., Immunohistochemistry (formalin/paraffin): 1:25 for 60 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 minutes followed by cooling at RT for 20 minutes., Optimal dilutions to be determined by the researcher. Positive Control: LS174T cells, breast carcinoma or tonsil. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.