Anti-CREB (cAMP Response Element Binding Protein)(48H2)

CREB is a bZIP transcription factor that activates target genes through cAMP response elements. CREB is able to mediate signals from numerous physiological stimuli, resulting in regulation of a broad array of cellular responses. While CREB is expressed in numerous tissues, it plays a large regulatory role in the nervous system. CREB is believed to play a key role in promoting neuronal survival, precursor proliferation, neurite outgrowth and neuronal differentiation in certain neuronal populations. Additionally, CREB signaling is involved in learning and memory in several organisms. CREB is able to selectively activate numerous downstream genes through interactions with different dimerization partners. CREB is activated by phosphorylation at Ser133 by various signaling pathways including Erk, Ca2+ and stress signaling. Some of the kinases involved in phosphorylating CREB at Ser133 are p90RSK, MSK, CaMKIV and MAPKAPK-2. Applications: Suitable for use in Western Blot, Immunoprecipation, Chromatin Immunoprecipitation, Immunohistochemistry, Immunofluorescence, Immunocytochemistry and Flow Cytometry. Other applications have not been tested. Recommended Dilutions: Western Blot: 1:1000. Incubate membrane with diluted antibody in 1X TBS, 5% BSA, 0.1% Tween-20 for 4ºC with gentle shaking overnight. , Immunoprecipitation: 1:250 , Chromatin Immunoprecipitation: 1:50, Immunohistochemistry (Paraffin): 1:6400, Use citrate as an unmasking buffer., Immunohistochemistry (Frozen): 1:6400 , Immunofluorescence (IC): 1:800 , Immunofluorescence (Frozen): 1:800, Flow Cytometry: 1:400 , Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.