Anti-COX 4-1 (COX IV-1)

Cytochrome C Oxidase (COX), which is located in the inner mitochondrial membrane, is the terminal enzyme complex of the mitochondrial electron transport chain. It collects electrons that are transferred from reduced Cytochrome C and donates them to molecular oxygen, which is then reduced to water. , Antibodies to this protein make an effective loading control for mitochondria. COXIV (COX4 )is generally expressed at a consistently high level. However, be aware that many proteins run at the same molecular weight as COXIV. Some caution is required when using antibodies to this protein as a loading control, as COXIV expression can vary under some manipulations. Cellular Localization: Mitochondrion, mito inner membrane , Positive Control: HEK 293 lysates, Hela lysates, Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.