Anti-COX-2 (Cyclooxygenase-2, PGHS 2, Prostaglandin Endoperoxide Synthase-2, PHS 2), FITC conjugated

Cyclooxygenase catalyzes the first step in the biosynthesis of prostaglandins, thromboxanes, and prostacyclins: the conversion of arachidonic acid to prostaglandin H2. Recent discoveries of the induction of cyclooxygenase biosynthesis by a variety of stimuli such as phorbol esters, lipopolysaccharides, and cytokines led to the hypothesis that the inducible form of cyclooxygenase, COX-2, is responsible for the biosynthesis of prostaglandins under acute inflammatory conditions. COX-2 is a 72kD protein which has been cloned from a variety of species including human, mouse, rat, and sheep. Applications: Suitable for use in Flow Cytometry and Immunocytochemistry. Not recommended for Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: Use 1ug antibody per 10e6 cells, Immunocytochemistry: 2-5ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. , Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.