Anti-COX 2 (Cyclooxygenase 2, PGHS 2, Prostaglandin Endoperoxide Synthase 2, PHS 2)

Prostaglandins are important regulators of immune responses, fever, and pain. Two isoforms of Prostaglandin H synthase are well characterized, namely COX1 (also called PGHS-1, PHS-1, Prostaglandin-endoperoxide synthase-1) and COX2 (also called PGHS-2, Prostaglandin-endoproxide synthase-2 and PHSII). Both forms of COX proteins are membrane associated heme proteins containing Cyclooxygenase and peroxidase activities. These enzymes are targets of NSAID (Non steroidal anti-inflammatory drugs) such as aspirin. Cox-1 (human 599 aa, rat 602 aa) is homodimer of 70KD subunits (1). COX1 is constitutively expressed although significant enhancement of COX1 expression can be induced in some cell types. High expression is observed in gastrointestinal tissues. COX2 is a 72KD protein having 60% homology to COX1. It is induced by cytokines and mitogens and is likely to play a role in inflammatory diseases such as rheumatoid arthritis. Applications: Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Other applications not tested. Recommended Dilution: Western Blot: 1:1000-1:5000 (ECL), ELISA: 1:10,000-100,000 Control peptide can be used to coat ELISA plates at 1ug/ml., Optimal dilutions to be determined by the researcher. Positive Control: 3T3 cells, BMMC, L-929, PC-12, C127 and CEF-147. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.