Anti-Complement C3, C3c (Complement Component C3c, C3/C3c, Acylation Stimulating Protein Cleavage Pr

C3 consists of two polypeptide chains (alpha, MW: 115kD and beta, MW: 75kD) and is usually present in serum at 1 to 2 mg/L. It is the pivotal protein of the complement system, being central in the classical pathway and the alternative pathway. It is cleaved by the classical pathway convertase C4b2a and the alternative pathway convertase C3bBb, into C3b and C3a. The C3a peptide is an important anaphylotoxin, whilst the C3b has three main roles: 1. Important opsonin phagocyte cells have C3b receptors. 2. Participates in the amplification loop in the alternative pathway. 3. Complexed with C4b2a this is the C5 convertase which leads ultimately to target cell lysis. C3c, along with iC3b and C3dg, is formed as a result of C3b inactivation by Factor I and various cofactors. The C3 fragments are eliminated by liver phagocytic cells. Applications: Suitable for use in Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry (Frozen): 1:50-1:100 using the Direct Method., Optimal dilutions to be determined by the researcher. Positive Control: Human skin, Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. FITC conjugates are sensitive to light.