Anti-Complement C1q (Complement Component 1, q Subcomponent)

Human complement component 1, q subcomponent (C1q) is a 156kD secreted protein. C1q associates with proenzymes C1r and C1s to form the calcium-dependent C1 complex, the first component of the serum complement system. C1q is composed of six A, six B-chains and six C-polypeptide chains. Each chain contains a collagen-like region located near the N-terminus and a C-terminal globular region. These regions bind the Fc region of IgM and IgG molecules, initiating the classical pathway of complement activation. C1q deficiency has been associated with lupus erythematosus and glomerulonephritis. Applications: Suitable for use in ELISA, Flow Cytometry, Immunofluorescence, Immunohistochemistry and Western Blot. Other applications have not been tested. Recommended Dilutions: Immunohistochemistry (Frozen): 1:500-1:1000, Optimal dilutions to be determined by the researcher. Positive Control: Kidney tissue from patients with streptococcal glomerulonephritis. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.