Anti-CNOT4 (CCR4-NOT Transcription Complex Subunit 4, CCR4-associated Factor 4, E3 Ubiquitin-protein

CNOT4 is a 575aa protein with E3 ubiquitin ligase activity, mainly involved in protein modification and protein ubiquitination. It contains a ring type zinc finger domain of the C4C4 type, an RNA recognition motif and a bipartite nuclear localization signal. Studies show that CNOT4 is a component of the CCR4-NOT (CAF1) core complex that functions as general transcription regulation complex and has both a positive as well as a negative effect on the transcription of multiple functionally unrelated genes, thus controls gene expression. CNOT4 interacts with CNOT1 via C-terminus and with E2 ubiquitin ligases via C4C4 RING domain. Reports suggest that CNOT4 gets phosphorylated in response to DNA damage on consensus sites recognized by ATM and ATR. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 0.05-0.1ug/ml, Optimal dilutions to be determined by the researcher. Positive Control: Human skeletal muscle lysate, Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.