Anti-Chromogranin A, clone 5G82

Chromogranin A (a protein of 439aa which is encoded on chromosome 14) is present in neuroendocrine cells throughout the body, including the neuroendocrine cells of the large and small intestine, adrenal medulla and pancreatic islets. It is an excellent marker for carcinoid tumors, phenochromocytomas, paragangliomas, and other neuroendocrine tumors. Coexpression of chromogranin A and neuron specific enolase (NSE) is common in neuroendocrine neoplasms. Comments: Excellent for staining of formalin/paraffin with no special pretreatment. , Applications: , Suitable for use in ELISA, Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry: (Formalin/paraffin): (Use Ab 1:200 for 30 min at RT) Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min., Optimal dilutions to be determined by the researcher. Positive Control: Pancreas , Cellular Localization: Cytoplasmic, Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.