Anti-Chorionic Gonadotropin, Human (hCG)

Human chorionic gonadotropin (hCG) is a glycoprotein hormone produced by placenta after conception. hCG is comprised of an a subunit with 92aa and a b subunit with 245aa. The a subunit is identical to that of LH (Luteinizing hormone), FSH (Follicle stimulating hormone) and TSH (Thyroid stimulating hormone). hCG sustains corpus luteum, stimulates progesterone secretion, hence is critical to early fetal development.  It has also been reported that hCG plays a role in the development of fetal tolerance to maternal immunity. hCG is the most sensitive biomarker for pregnancy. Applications: Suitable for use in ELISA, Purification of HCG by Affinity Chromatography, Qualitative and Quantitative Immunoprecipitation. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Hybridoma: Sp2/0-Ag14 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.