Anti-CEBPA (CCAAT/Enhancer-binding Protein alpha, C/EBP alpha)

CEBP alpha (CCAAT/Enhancer-Binding Protein? also C/EBP) is a 42-43kD member of the C/EBP subfamily, bZIP family of proteins. It is expressed in multiple cell types, particularly those that are fully differentiated, and includes hepatocytes, skeletal muscle cells, adipocytes, intestinal epithelium and proliferating macrophages. CEBP alpha acts as a transcriptional activator for a large and diverse number of genes, including glycogen synthase, GLUT4, and G-CSFR, and participates in both cell cycle progression and differentiation. Human CEBP a is 358aa in length. It contains one transactivation domain (aa1-96), poly-Gly segment (aa99-104), a polyPromotif (aa 183-189), a bZIP dimerization region (aa281-334), and an overlapping DNA binding Leu-zipper domain (aa317-345). CEBP alpha forms both homodimers, and heterodimers with other subfamily members. One alternative start site at 120aa generates a low activity 30-32kD isoform variant. There is also a utilized phosphorylation site at 21aa that regulates activity. Over aa1-124, human CEBP alpha shares 93% aa identity with mouse CEBP alpha. Applications: Suitable for use in Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry: 5-15ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.