Anti-cdk4, p34 (CDK4, Cyclin-dependent Kinase 4, p34cdk4), clone 0.N.199

A family of proteins designated as cdks are critical regulators of cell cycle progression. The prototype member of this family, p34cdc2, and a related protein cdk2, function late in the cycle while cdk4 and cdk6 are critically involved in G1 to S progression. Applications: Suitable for use in Immunofluorescence, Immunoprecipitation, Western Blot and Kinase assays. Other applications have not been tested. Recommended Dilution: Immunoprecipitation Native only, coprecipitates cyclin D's: 2ug/mg protein lysate. Use protein G, Western Blot: 1-2ug/ml for 2 hours at RT., Optiml dilutions to be determined by the researcher. Positive Control: MAD109, NIH3T3, LS174T, and MCF-7 cells. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.