Anti-cdc2, p34

Applications: , Suitable for use in ELISA, Western Blot, Immunoprecipitation, Inhibition and Immunohistochemistry. Other applications not tested. Recommended Dilution: ELISA: 0.1-1ug/ml , Western Blot: 0.5-1ug/ml , Immunoprecipitation: 5ug. Capable of immunoprecipitating cyclin protein associated with p34cdc2., Inhibition: Inhibits the activation of p34cdc2 kinase by cyclin proteins. , Immunohistochemistry (Frozen): 5-10ug/ml., Optimal dilutions to be determined by the researcher. Positive Control: Western Blot: A431 and HeLa 3T3 cell lysates, Xenopus egg extracts., Immunohistochemistry: Tested on a variety of human tissues including tonsil (germinal center), spermatogonia and skin (basal layer). Epitope Location: The epitope core residues are thought to reside within amino acids 220-227 of the mouse p34cdc2 protein corresponding to the sequence LGTPNNEV. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.