Anti-CDC2 (Cell Division Protein Kinase 1, Cell Division Control Protein 2 Homolog, Cyclin-dependent

CDC2 is a catalytic subunit of a protein kinase complex, called the M-phase promoting factor, that induces entry into mitosis and is universal among eukaryotes. In HeLa cells CDC2 is the most abundant phosphotyrosine-containing protein and its phosphotyrosine content is subject to cell cycle regulation. CDC2 gene is located on chromosome 10. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 0.5-1ug/ml, Immunohistochemistry: 1-2ug/ml detects CDC2 in formalin fixed and paraffin embedded tissues and formalin or acetone fixed frozen tissues., Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile 1.2% sodium acetate or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.