Anti-cdc14B Phosphatase

Cdc14, also known as duel specificity phosphatase, is a protein phosphatase originally discovered in yeast that plays an important role in cell cycle regulation, including exit from mitosis (1). Throughout G1, S and G2 phases, Cdc14 is located in the nucleolus, at the beginning of anaphase, the protein is redistributed throughout the entire nucleus (2). In late anaphase, Cdc14 dephosphorylates Cdh1, which then activates APC (Anaphase Promoting Complex) (3). , Two human orthologs of yeast Cdc14 have been identified, these are Cdc14A and Cdc14B, and they share about 80% amino acid homology in the N-termini and phosphatase domains (4). Cdc14 has also been shown to dephosphorylate p53, suggesting that it may play a role in regulation of p53 activity. Applications: Suitable for use in Immunoprecipitation and Western Blot. Other applications not tested. Recommended Dilution: Immunoprecipitation: 1-3ug/ml, Western Blot: 1-3ug/ml, Optimal dilutions to be determined by the researcher. Positive Controls: Human HeLa cell lysates, Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.