Anti-CD94 (Natural Killer Cells Antigen CD94, KP43, Killer Cell Lectin-like Receptor Subfamily D Mem

CD94 is a 27-31kD glycoprotein member of the C-type lectin superfamily. It is expressed on NK cells, particularly those that are CD56bright, plus those that are CD56dim but secrete material amounts of IFN-G. CD94 forms covalent transmembrane (TM) heterodimers with NKG2A, B, C, E and H, and binds HLA-E (in human, Qa-1b in mouse) as part of a cellular homeostatic monitoring system. Amide-linkage based complexes with NKG2A are predicted to run at 70-80kD in SDS-PAGE. Depending upon the exact NKG2 partner, CD94 ligation can result in either NK cell activation (2C, 2H or 2E) or inhibition (2A or 2B). Human CD94 is a 179aa type II TM protein. It possesses a short cytoplasmic segment aa1-10 plus a 158aa extracellular region aa32-179 that contains one C-type lectin domain aa61-176. Multiple splice variants exist. One is a 149aa, 19kD cytosolic protein that shows an Ala substitution for aa3-34, while a second is a truncated, 104aa, 17kD TM protein. A third isoform is 158aa in length and 25kD in size, and shows a deletion of aa34-54. Over aa32-179, human CD94 shares 53% aa identity with mouse CD94. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.