Anti-CD86 (B7-2), FITC conjugated, clone 5K50

CD86 is the B7-2 costimulatory molecule and a ligand for CD28 and CD152. CD86/B7-2 is an 80kD type I integral membrane protein and a member of the immunoglobulin superfamily of cell surface receptors. Along with CD80/B7-1, CD86 participates in T cell costimulation via interaction with CD28 and CD152/CTLA-4. It is expressed at very low density in normal tissues but is rapidly upregulated on stimulated B cells, monocytes and dendritic cells. Since CD86/B7-2 has rapid kinetics of induction, it is believed to be the major CD28 ligand expressed early in the immune response. Applications:  Identification and enumeration of CD86+ cells by flow cytometry 1, 3, 4, 9,  Identification of CD86+ cells in frozen sections 8, 9,  Immunoprecipitation 3,  In vivo and in vitro blocking assays 1, 2, 8, 9, Recommended Dilution: Flow Cytometry: < 1ug/10e6 cells, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.