Anti-CD71 (Transferrin Receptor, TR, Tfr, TfnrR) (Biotin)

Human CD71 is a type II transmembrane glycoprotein found on activated leukocytes. CD71 binds transferrin and mediates uptake of iron through endocytosis and recycling. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 5ug/ml, Immunohistochemistry: frozen sections, Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. 5x10e5 cultured human HPB-MLT cells were washed and incubated for 45 minutes on ice with 80ul of C2426-02B at 5ug/ml. , 2. Cells were washed twice and incubated with Streptavidin/R-Phycoerythrin (S7973-88P)., 3. Cells were washed three times, fixed and analyzed by FACS. , 4. Cells stained positive with a mean shift of 1.9 log10 fluorescent units when compared to a Mouse IgG1/Biotin negative control at a similar concentration. , 5. Binding was 97% blocked when cells were pre-incubated with 20ul of 0.5mg/ml C2426-02A. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer., ,