Anti-CD54 (Intercellular Adhesion Molecule, ICAM-1) (PE)

Human CD54 (ICAM-1) mediates cell adhesion by binding to the integrins CD11a/CD18 (LFA-1) and to CD11b/CD18 (Mac-1). CD54 expression on resting peripheral blood leukocytes is weak but is upregulated on activated T and B lymphocytes and monocytes. Applications: Suitable for use in FLISA and Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:50 (80ul/test), Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. Five x 10e5 cultured Raji cells were washed and incubated 45 minutes on ice with 80ul of C2409-10H at a 1:50 dilution. 2. Cells were washed three times, fixed and analyzed using a BD FACstar plus. , 3. Cells stained positive with a mean shift of 2.47 log10 fluorescent units when compared to a Mouse IgG1/R-PE negative control at a similar concentration. , 4. Binding was 91% blocked when pre incubated for 10 minutes with 20ul of 0.5mg/ml C2409-10E. Storage and Stability: May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only pror to immediate use. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity.