Anti-CD54 (Intercellular Adhesion Molecule, ICAM-1), FITC conjugated, clone 6A320

CD29 forms non-covalent bonds with the integrin alpha subunits, including CD51 and CD49a-f, to form heterodimers. The ligands for these heterodimers include collagen, fibronectin, laminin and vascular adhesion molecule-1. In the immune system beta 1 integrins play an important role in cell adhesion, migration, activation and differentiation., CD29 is reported to inhibit beta 1 integrin mediated adhesion (1). Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:25-1:100, Use 10ul to label 10e6 cells in 100ul., Optimal dilution determined by the researcher, Hybridoma: Spleen cells from immunized BALB/c mice were fused with cells from the PAI mouse myeloma cell line. Recommended Negative Controls: IgG1 Negative Control: FITC , Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. , Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.