Anti-CD54 (Intercellular Adhesion Molecule, ICAM-1), clone 6A320

CD54 cell surface antigen, also known as intercellular adhesion molecule-1 (ICAM-1), is a 90kD adhesion molecule belonging to the immunoglobulin superfamily. The rat ICAM-1 molecule has an important role in various cell-cell interactions in the immune system. IP analysis shows that the antigen has the same features as human ICAM-1. In addition, recent studies suggest that cross-linking of ICAM-1 induces calcium signalling. Applications: Suitable for use in Flow Cytometry, Immunohistochemistry, Immunofluorescence and Immunoprecipitation. Other applications not tested. Recommended Dilution: Flow Cytometry: Neat, 1:25-1:50. Use 10ul to label 10e6 cells in 100ul., Immunohistochemistry: frozen, Optimal dilution determined by the researcher, Hybridoma: Spleen cells from immunized BALB/c mice were fused with cells from the PAI mouse myeloma cell line. Recommended Negative Controls: I1904-78Y: IgG1, Murine Negative Control (For rat tissue), Recommended secondary antibodies: (Rat Adsorbed) I1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo, I1904-06F: IgG, H&L (FITC) (X-Adsorbed) Pab Gt xMo, Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.