Anti-CD49b (Integrin alpha2, ITGA2, alpha-2 subunit of VLA-2 Receptor, Platelet Antigen B), FITC con

Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. The I-domain containing alpha integrin 2 combines with beta integrin 1 to form a collagen-binding integrin referred to as glycoprotein Ia/IIa (gpIa/IIa) when expressed on platelets, and very late (activation) antigen 2 ('VLA-2') when found on T-cells. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling. CD49b is expressed approximately 50% of monocytes and most adherent cell lines. CD49b forms a non-covalent association with the 130kD VLA beta chain (CD29) in the VLA-2 complex. The CD49b/CD29 complex is also known as a2/b1 and VLA-2. The complex mediates binding of cells to collagen (types I, II, III, and IV) and to laminin. The complex of CD49b associated with CD29 (integrin b1) is a receptor for laminin and collagen. This complex is expressed by some CD4-positive T-cells, natural killer cells, epithelial cells and platelets. , Applications:, Suitable for use in Flow Cytometry. Not suitable for use in Immunohistochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:50-1:100. Use 10ul of the suggested working dilution to label 10e6 cells in 100ul. Optimal working dilutions to be determined by researcher. Recommended Negative Controls: Mouse IgG1 Negative Control, Fusion Partners: Spleen cells from immunized mice were fused with cells of the mouse SP2/0 myeloma cell line. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. , Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.