Anti-CD49b (Integrin alpha2, ITGA2, alpha-2 subunit of VLA-2 Receptor, Platelet Antigen B) (FITC)

Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. The I-domain containing alpha integrin 2 combines with beta integrin 1 to form a collagen-binding integrin referred to as glycoprotein Ia/IIa (gpIa/IIa) when expressed on platelets, and very late (activation) antigen 2 ('VLA-2') when found on T-cells. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling. CD49b is expressed approximately 50% of monocytes and most adherent cell lines. CD49b forms a non-covalent association with the 130kD VLA beta chain (CD29) in the VLA-2 complex. The CD49b/CD29 complex is also known as a2/b1 and VLA-2. The complex mediates binding of cells to collagen (types I, II, III, and IV) and to laminin. The complex of CD49b associated with CD29 (integrin b1) is a receptor for laminin and collagen. This complex is expressed by some CD4-positive T-cells, natural killer cells, epithelial cells and platelets. , Fusion Partners: Spleen cells from immunized mice were fused with cells of the mouse NSI myeloma cell line. FITC: Protein (molar ratio): 14.2: 1 , Recommended Negative Controls: , Mouse IgG1 Negative Control: FITC, Applications: Suitable for use in Flow Cytometry. Flow Cytometry: Neat. Use 10ul of the suggested working dilution to label 10e6 cells or 100ul whole blood.Optimal working dilutions to be determined by researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.