Anti-CD4 (Tac antigen, p55, Interleukin 2 Receptor alpha, IL2Ra, T Cell Growth Factor Receptor), clo

CD4, a single chain transmembrane glycoprotein is found on a T cell subset (helper/inducer) representing 45% of peripheral blood lympocytes. It is also present on 80% of thymocytes and at a lower level on monocytes. It is involved in recognition of antigen presented along with MHC class II by APCs. It serves as receptor for HIV. Antibody to CD4 recognizes T-helper cells required for recognition of class II antigens. It reacts with 60% of peripheral blood E rosette-positive (E+) cells while showing negligible reactivity with E+ cells, monocytes, granulocytes, EBV-transformed B cell lines, and mouse splenocytes., CD4-positive T-cells provide helper funciton in pokeweed mitogen driven B-cell differentiation. Moreover, anti-CD4 selectively blocks the autologus mixed lymphocyte reaction (AMLR) with minimal or no effect on the allogeneic MLR (allo-MLR). Blocking of the autoreactivity occurs when either autologus B-lymphocytes or macrophages are used as stimulators. Specificity closely approximates the binding spectrum of OKT4 and Leu-3 antibodies both of which also recognize 55 to 65% of human peripheral blood T-cells. Applications: Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications have not been tested. Recommended Dilution: Flow Cytometry: 1:10-1:50. Use 10ul label 10e6 cells in 100ul. , Immunohistochemistry: Frozen, Optimal working dilutions to be determined by researcher. Recommended Secondary Reagents: Goat anti-mouse IgG: HRP conjugate (non-cross reacting with rat IgG), IgG Goat anti mouse IgG: RPE (rat adsorbed), Hybridoma: Spleen cells from immunized BALB/c mice were fused with cells of the mouse NS1 myeloma cell line, Recommended Negative Controls: Mouse IgG2a Negative Control, Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.