Anti-CD4 (L3T4) (Tac antigen, p55, Interleukin 2 Receptor alpha, IL2Ra, T Cell Growth Factor Recepto

CD4/L3T4 is a type I transmembrane glycoprotein expressed on most thymocytes and the helper/inducer subpopulation of mature T lymphocytes of all mouse strains tested (1,2). CD4 has also been detected at low density on pluripotent hematopoietic stem cells, bone marrow myeloid precursors, and intrathymic lymphoid precursors (3-6). The CD4 antigen functions as a co-receptor, interacting with class II major histocompatability complex (MHC) molecules in the recognition of foreign antigens by T cells (1,2). In vivo and in vitro treatment with the effectively depletes CD4+ cells (7,8). The mAb also blocks helper T cell responses to MHC class II antigens, including cytolysis, proliferation, allogeneic B cell help, and release of lymphokines (1,8-13). Applications: Suitable for use in Flow cytometry, Immunohistochemistry, Immunoprecipitation, In vivo and in vitro depletion of CD4+ cells, Inhibition of T helper function and in Fractionation of CD4+ cells. Other applications have not been tested. Recommended Dilution: Flow Cytometry: <1ug/10e6 cells, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. ,