Anti-CD35, C3b (CR1, Follicular Dendritic Cell Marker), clone 1.BB.342

CD35 antigen is composed of 4 allotypes. C3b Receptor (CR1) is present on the following: B lymphocytes, Monocytes, Myeloid cells, Dendritic cells of lymphocytes follicules, Renal podocytes. Applications: Suitable for use in degradation of C3b, analysis of lymphoid, myeloid differentiation, Fluorescence Microscopy, Flow Cytometry, and Immunohistochemistry. Other applications have not been tested. Recommended Dilution: Flow Cytometry: 2ug/5x10e5 cells/test. , Immunohistochemistry: 1:25-1:50. Frozen sections or cell smears and on Bouin's ?xed, parrif?n-embedded tissues., Optimal dilutions to be determined by the researcher. Hybridoma: Hybridization of NS 1myeloma cells with spleen cells from Balb/c mice. Storage and Stability: Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol, aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.