Anti-CD273 (B7-DC, Programmed Death Ligand 2, PD-L2)

CD273 is a single-pass type 1 membrane protein belonging to the immunoglobulin receptor superfamily, and is a ligand for CD279 (PD-1). CD273 is highly expressed in the tissues of the heart, liver, lung, placenta and pancreas. It is expressed in dendritic cells, and its expression can be induced on inflammatory macrophages when activated by interleukin 4 or Th2 cells. CD273 functions to inhibit T-cell proliferation by blocking cell cycle progression and cytokine production, and is particularly important in the maintenance of self tolerance. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 0.5-1ug/ml, Immunohistochemistry (Paraffin): 2.5ug/ml. Antigen retrieval by heating in sodium citrate buffer, pH 6.0 is required., Optimal dilutions to be determined by the researcher, Positive Control Tissue (IHC): Mouse brain, Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.