Anti-CD154 (CD40 Ligand, CD40L, TRAP)

CD40L is a transmembrane cell surface glycoprotein of 39kD expressed mainly on CD4+ T-cells and platelets in an activation-dependent manner. The interaction of CD40L on activated CD4+ T-cells with CD40 on antigen presenting cells is essential for the T-cell-dependent humoral immune respons. It has a role in T-cell-dependent Bcell proliferation, differentiation and memory cell formation. Blocking of the CD40-CD40L interaction results in immunoglobulin isotype switch. Defects in CD40L are the cause of X-linked immunodeficiency with hyper-IgM type Ig which is characterized by elavated concentrations of serum IgMs and decreased amounts of all other types. In vivo, interruption of the CD40 signalling system limits experimental autoimmune diseases such as collagen-induced arthritis, lupus nephritis, rheumatoid arthritis, systemic lupus, erythematosus (SLE) and acute or chronic graft-versus-host disease. CD40/CD40L is involved in angiogenic processes. In this process activated platelets are the source of CD40L for CD40 expressed by endothelial cells. Applications: Suitable for use in Immunoassays, Western Blot, Immunoprecipitation, Flow Cytometry, and neutralization of biological activity. Other applications not tested. Recommended Dilutions: Flow Cytometry: 1:50, Western Blot: 1:50, Optimal dilution to be determined by researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.