Anti-CD137 (4-1BB), clone 3H1858

A glycoprotein (256 amino acids) of 30kD, which exists as both a monomer and a 55kD dimer on the surface of activated T cells is identified as 4-1BB (also known as CD137). 4-1BB is an inducible receptor-like protein expressed on the cell surface of activated splenic T cells and thymocytes. 4-1BB is structurally related to the members of NGFR/TNFR superfamily which are characterized by the presence of three-six patterns of a cysteine-rich motif in their extracellular domains. Other members of this family include low affinity NGFR, two receptors for TNF (TNFR-I & TNFR-II), CD30, CD40, OX40, Fas, and CD27. These molecules are involved in cell growth, survival, and death processes. The cytoplasmic domain of 4-1BB include two runs of acidic amino acids, a potential p56lck binding site, five consecutive glycines at the C-terminus, and four potential phosphorylation sites: one tyrosine, two threonine, and one serine. The cytoplasmic domain of 4-1BB displays sequence homology to CD4 and CD8 and is responsible for the binding of 4-1BB to p56lck, a protein tyrosine kinase involved in T cell proliferation. Applications: Flow Cytometry: 1:10-1:50. Use 10ul of the suggested working dilution to label 10e6 cells in 100ul. Optimal working dilutions to be determined by researcher. Recommended Secondary Antibody: F(ab')2 Goat anti-rat IgG: RPE (mouse adsorbed), Recommended Negative Controls: Rat IgG2a Negative Control, Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.