Anti-CD109 (CD109 Antigen, 150kD TGF-beta-1-binding Protein, C3 and PZP-like alpha-2-macroglobulin D

CD109 is a GPI-anchored member of the A2-macroglobulin (A2M) and complement family of proteins. Mature human CD109 contains a bait region with recognition sequences for multiple proteases, an internal thioester bond, and a domain similar to the receptor binding domain of A2M. Cleavage of A2M family proteins within the bait region activates the thioester bond to promote covalent bonding to nucleophilic groups in adjacent molecules. Within the region included in the recombinant protein used for immunization, human CD109 shares 71%-73% aa sequence identity with mouse and rat CD109. CD109 is expressed on activated T cells and platelets, hematopoietic stem cells, megakaryocyte precursors, vascular endothelial cells, basal and myoepithelial cells of secretory glands, and squamous cell carcinomas. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 10ul labels 1x10e6 cells using A431 human epithelial carcinoma cell line and mouse splenocytes treated with PMA and calcium ionomycin, Optimal dilutions to be determined by the researcher. Excitation: 488nm , Emission: 565-605nm, Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.