Anti-CD106 (Vascular Cell Adhesion Molecule-1, VCAM-1, INCAM-110), Phycoerythrin conjugated, clone 1

VCAM-1 is an adhesion molecule and a major mediator of the inflammatory response. It is expressed on activated microvascular endothelial cells in response to signals arising from immune responses in infection, graft rejection, tumor recognition and killing. The complementary binding ligand for VCAM-1 is VLA-4/CD49d. In addition to VCAM-1, VLA-4 also recognizes the extracellular matrix molecule fibronectin. This pairing of VCAM-1 and VLA-4 is able to provide a second signal (i.e., non-antigen specific) for T cell stimulation, such as that seen in transplantation. The monoclonal antibody has been used in transplant studies to suppress cardiac rejection and induce long-term cardiac graft survival. In addition to inflammatory responses, VCAM-1 has a significant role in hemopoiesis through its ability to retain lymphocyte and myeloid precursors on stromal cells in the marrow and lymphoid organs. CD106/VCAM-1 exists as an integral membrane protein. The monoclonal antibody immunoprecipitates a peptide that gives a single band on SDS-PAGE gels with an apparent Mr of ~100kD under reducing conditions and 92kD under nonreducing conditions (1-5). Applications: Suitable for use in Immunofluorescence, Flow Cytometry, Immunoprecipitation, Immunohistochemistry., Other applications not tested. Recommended Dilution: Flow Cytometry: <0.2ug/10e6 cells, Optimal dilutions to be determined by the researcher. Storage and Stability: Storage and Stability:, May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity., , Note: We do not recommended adding glycerol as a cryopreservative.