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Item number | Size | Datasheet | Manual | SDS | Delivery time | Quantity | Price |
---|---|---|---|---|---|---|---|
C2446-50E1.100 | 100 µg | - | - |
3 - 19 business days* |
621.00€
|
If you have any questions, please use our Contact Form.
You can also order by e-mail: info@biomol.com
Larger quantity required? Request bulk
You can also order by e-mail: info@biomol.com
Larger quantity required? Request bulk
Human CD105 (endoglin) is a homodimeric membrane glycoprotein virtually restricted to endothelial... more
Product information "Anti-CD105 (Endoglin, gp160, TGF beta1,3 Receptor) (Biotin)"
Human CD105 (endoglin) is a homodimeric membrane glycoprotein virtually restricted to endothelial cells, and present on some leukemia cells and minor subsets of bone marrow cells. CD105 binds to TGF-b1 and b3 with high affinity. Antibody will augment binding of TGF-b1 to CD105 expressing leukemia cells, Applications: Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. 5x10e5 Nalm-6 were washed and incubated 45 minutes on ice with 80ul of C2446-50E1 at 5ug/ml., 2. Cells were washed twice and incubated with secondary reagent S7973-88P, Streptavidin/R-Phycoerythrin, after which they were washed three times fixed and analyzed using a FACS. , 3. Cells stained positive with a mean shift of 1.81 log10 fluorescent units when compared to I1904-78R2 IgG1/biotin Murine Negative Control at a similar concentration. , 4. Binding was 99% blocked when cells were preincubated 10 minutes with 20ul of 0.5mg/ml C2446-50E, CD105. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Keywords: | Anti-ENG, Anti-END, Anti-CD105, Anti-Endoglin |
Supplier: | United States Biological |
Supplier-Nr: | C2446-50E1 |
Properties
Application: | FC, IHC |
Antibody Type: | Monoclonal |
Clone: | 7H217 |
Conjugate: | Biotin |
Host: | Mouse |
Species reactivity: | human |
Immunogen: | Membrane preparation of human B-lineage leukemia cells |
Format: | Affinity Purified |
Database Information
KEGG ID : | K06526 | Matching products |
UniProt ID : | P17813 | Matching products |
Gene ID | GeneID 2022 | Matching products |
Handling & Safety
Storage: | +4°C |
Shipping: | +4°C (International: +4°C) |
Caution
Our products are for laboratory research use only: Not for administration to humans!
Our products are for laboratory research use only: Not for administration to humans!
Information about the product reference will follow.
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