Anti-Cathepsin S (Cathepsin-S, CATS, CTSS, FLJ50259, MGC3886)

Applications:, Suitable for use in Immunohistochemistry. Other applications not tested. Recommended Dilutions: Immunohistochemistry (Formalin-fixed paraffin): 1:500, Optimal dilutions to be determined by the researcher. Immunohistochemistry Protocol: Tissue Sample: Mouse and rat visceral fat tissue, Fixative: 10% Formalin, Embedding: Paraffin, Negative control: Pre-immuno serum, Pretreatment: Intact, Blocking: 2% Normal Goat Serum, Primary Antibody: C2097-77D, Optimal Dilution: 1:500 (1 hour at RT), Secondary Antibody: IgG (Biotin) GtxRb (1:400, 30 min), Amplification: ABC (Vector) (1:400, 30 min), Detection system: HRP, Substrate: DAB, 3 minutes, Counterstained: Hematoxylin, 30 seconds, Amino Acid Sequence: Asn-Ser-Trp-Gly-His-Asn-Phe-Gly-Glu-Glu-Gly-Tyr-Ile-Arg-Met-Ala-Arg-Asn-Lys-Gly-Asn-His-Cys-Gly-Ile-Ala-Ser-Phe-Pro-Ser-Tyr-Pro-Glu-Ile, Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.