Anti-Cathepsin L (Cathepsin L heavy chain, Cathepsin L light chain, CATL, CTSL, Major excreted prote

Cathepsins are lysosomal proteases that play an important role in the intracellular degradation of exogenous and endogenous proteins, activation of enzyme precursors, and tumor invasion and metastasis. Normally located in lysosomes of almost all mammalian cells, they can be secreted from the cell under certain conditions. Cathepsin L is responsible for most of the intralysosomal breakdown of normal cells. Cathepsin L is secreted by numerous transformed cells in its inactive proform, and mRNA expression level seems to correlate with their metastatic potential. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry and Functional Studies. Other applications not tested. Recommended Dilution: ELISA: 1:200, Immunohistochemistry: frozen sections, Functional Studies: Suppresses the growth of malignant myeloma cells., Optimal dilutions to be determined by the researcher. Positive Control: Whole cell extract from cultured mink lung cell line. , Storage and Stability:, May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.