Anti-Caspase-9 (ICE-LAP6, Mch6, Apaf 3), clone 4E31

Caspase-9/mammalian Ced-3 homologue, also known as ICE-LAP6, Mch6 and Apaf-3, is a member of the Ced3/ICE cysteine protease caspase family that has a predicted molecular mass of 46kD and was originally isolated in human Jurkat T cells (1). Caspase-9 is critical for cytochrome c-dependent apoptosis and normal brain development (3). The active form of caspase-9 is composed of a 32-35kD subunit and a 10kD subunit that are proteolytically derived from procaspase-9 by caspase-3 (CPP3) and granzyme B (2). Procaspase-9 activation involves formation of a complex with the cofactor Apaf-1, cytochrome C, and deoxyadenosine triphosphate. Once activated, caspase-9 initiates a caspase cascade involving caspase-3,-6, and-7. Caspase-9 is expressed in a variety of tissues. The small heat shock protein Hsp27, has been shown to inhibit etoposide-induced apoptosis by preventing cytochrome C and dATP-triggered activity of caspase-9 (4). Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications have not been tested. Recommended Dilutions: Western Blot (ECL): 2ug/ml , Immunoprecipitation: 12.5ug/ml , Optimal dilutions to be determined by researcher., , Positive Controls:, HeLa Cell Lysate, Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.