Anti-Caspase-9 (ICE-LAP6, Mch6, Apaf 3)

Caspase-9 (ICE-LAP6, Mch6) is an important member of the cysteine aspartic acid protease (caspase) family (1,2). Upon apoptotic stimulation, cytochrome c released from mitochondria associates with procaspase-9 (47kD)/Apaf 1. This complex processes procaspase-9 into a large active subunit (35kD or 17kD) and a small subunit (10kD) by self-cleavage at Asp315 (3-5). Cleaved caspase-9 further processes other caspase members, including caspase- 3 and caspase-7, to initiate a caspase cascade, leading to programmed cell death (6-9). In addition to self-cleavage, procaspase-9 also can be cleaved in vivo by caspase-3 at Asp330. This process serves as positive feedback to amplify the apoptotic signal in the caspase activation pathway (3-5). Recommended Dilution: ELISA, Western Blot: 1:1000, Molecular Weight: 17, 35, 37, 47kD, Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.