Anti-Caspase-9 (ICE-LAP6, Mch6, Apaf 3)

Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. A novel member in the caspase family was recently identified and designated ICE-LAP6, Mch6, and Apaf-3 (1-3). Caspase 9 and Apaf-1 bind to each other, which leads to Caspase 9 activation (3). , Caspase 9 is also activated by granzyme B and CPP32 (1,2). Activated Caspase 9 cleaves and activates Caspase 3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis (3). Caspase 9 plays a central role in cell death induced by a wide variety of apoptosis activators including TNFalpha, TRAIL, anti-CD95, FADD and TRADD (4). Caspase 9 is expressed in a variety of human tissues (1,2). Applications: Suitable for use in Western Blot. Other applications have not been tested. Recommended Dilution: Western Blot: 0.5-4ug/ml, Optimal dilutions to be determined by researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.