Anti-Caspase-1 (Interleukin-1Beta Converting Enzyme, ICE)

Caspase 1/ICE (Interleukin-1b Converting Enzyme) is expressed as a proenzyme of 45kD in many tissues. Upon stimulus it gets activated by proteolytic cleavage. Active Caspase 1 is a tetramer of two subunits p10 and p20 in 2,2 proportion. Caspase 1 (cystein aspartic-specific protease 1) cleaves its substrates after an aspartate residue. It cleaves Interleukin b into its active form which further exerts its proinflammatory effects. Overexpression of caspase 1 can induce apoptosis in fibroblast which can be inhibited by overexpression of Crm A, a protein from pox virus. Applications: Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 0.5-4ug/ml, Immunocytochemistry: 10-20ug/ml, Immunoprecipitation: 4-8ug/ml, Immunohistochemistry: 1:50, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.