Anti-Carbonic Anhydrase IX (CA9, Membrane Antigen MN, Renal Cell Carcinoma (RCC)-associated Antigen

Carbonic Anhydrase (CA) catalyzes the reversible reaction of CO2 + H2O = HCO3- + H+, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1­3). Topics in the CA meeting (6th International Conference on the CAs, June 20­25, 2003, Slovakia) ranged from use of CAs as markers for tumor and hypoxia in clinic, as nutritional supplement in milk, and as a tool for CO2 removal and mosquito control in industry. CA9, also known as membrane antigen MN and renal cell carcinoma (RCC)­associated antigen G250, is a transmembrane enzyme expressed primarily in carcinoma cells. It is one of the best markers for hypoxia and for RCC (4, 5). rhCA9 corresponds to the extracellular portion of human CA9. Applications: Suitable for use in ELISA, Flow Cytometry and Immunoprecipitation. Other applications not tested. Recommended Dilutions: ELISA (Capture): 2-8ug/ml, Flow Cytometry: 2.5ug labels 10e6 cells using the HeLa human cervical epithelial carcinoma cell line., Immunoprecipitation: 25ug/ml using conditioned culture medium spiked with recombinant human Carbonic Anhydrase IX., Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Reconstitute with sterile PBS. Aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.