Anti-Carbonic Anhydrase III (CA3, arbonic anhydrase III, muscle specific CAIII, CA-III, Car3, Carbon

Carbonic Anhydrase is a group of metalloproteases that catalyze the reversible reaction of CO2 + H2O = HCO3- + H+, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption. CA3 is a cytosolic enzyme with a very low CA activity. It is present at high levels in skeletal muscle and much lower levels in other tissues, such as cardiac and smooth muscle. The aa sequence of human CA3 is 99%, 90% and 92% identical to that of chimpanzee, dog and mouse/rat. Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 2ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile 40-50% glycerol, PBS.. Aliquot and store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.