Anti-Carbohydrate Sulfotransferase 5 (CHST5, Carbohydrate (N-acetylglucosamine 6-O) sulfotransferase

CHST5 (Carbohydrate sulfotransferase 5, also GlcNAc6ST-3, I-GlcNAc6ST and GST4 alpha) is likely a 53-56kD member of the sulfotransfease family of enzymes. It is a Golgi-embedded enzyme that is found in T cells, B cells and intestinal epithelium. It appears to act only on short carbohydrates and transfers sulfate to position #6 of mucin-associated GlcNAc. Human CHST5 is a 390aa type II transmembrane protein. It contains a short cytoplasmic segment (aa1-9) and a long luminal domain (aa28-390) that contains an enzymatic region (aa180-358). There is , one alternate start site that is 22aa upstream of the standard site. Humans have a GST4-alpha and -beta gene, the result of gene duplication. Over aa28-390, these two gene products share 87% aa identity. Mouse has only one GST4 gene, and it is functionally related to human GST4- beta. Over aa 28-390, human GST4-alpha/CHST5 is 78% aa identical to mouse GST4. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 0.5-1.0ug/ml, Western Blot: 1.0ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile 40-50% glycerol, PBS.. Aliquot and store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.