Anti-C1q Complement, human, clone 5F91

C1q is serum glycoprotein of 18-polypeptides chains consisting of three non-identical subunits, A (29kD), B (26kD) and C (246aa, 19kD) in molar ratio of 1:2:2. C1Q in the plasma is complexed with two proenzymes C1r and two C1s molecules to form the first component of complement (C1). Activation of complement via classical pathway is triggered by binding of globular head of C1q to immune complexes containing IgG (Fc-region) or IgM or to a variety of other activating substances, including C-reactive protein, retrovirus and mitochondria. Subsequent to C1q binding, c1r and C1s are converted to proteolytic enzymes that are responsible to continuation of activation via the classical pathway. Normal plasma concentration of C1q is 100-180mg/L. Applications: Suitable for use in ELISA, Western Blot and Inhibition experiments (e.g. hemolytic activity). Other applications have not been tested. Recommended Dilutions: Western Blot: 1:100 (ECL method), Incubation Time 1hr at RT., Inhibition of hemolytic activity: 0.85ug/ml inhibits 50% of hemolytic activity in the fluid phase. With C1 bound to immune complexes a dose dependent increase of C1 activity was observed at antibody concentrations of 1.5ug/ml. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.