Anti-Bromodomain and PHD Finger Containing 1 (BRPF1, BR1

The protein encoded by this gene is expressed ubiquitously and at the highest level in testes and spermatogonia. The protein is localized within nuclei, and it is very similar in structure to two zinc finger proteins, AF10 and AF17. It is suggested that these proteins form a family of regulatory proteins. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene. Applications: Suitable for use in Flow Cytometry, ELISA, Western Blot, Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 0.2-2ug/ml, Immunoprecipitation: 100-500ug, Flow Cytometry: 0.5-2ug, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.